Molecular genetic characterization of the RD-114 gene family of endogenous feline retroviral sequences
نویسندگان
چکیده
منابع مشابه
Characterization of enhancer elements and their mutations in the long terminal repeat of feline endogenous RD-114 proviruses.
To locate the enhancer regions of the feline endogenous RD-114 long terminal repeat (LTR), we examined expression of the chloramphenicol acetyltransferase gene driven by various segments of the U3 region from two different proviral loci (CRL3 and CR1). Transient expression assays demonstrated that the primary signal sequence for transcription enhancement was located within the 63-base-pair (bp)...
متن کاملExperimental infection of dogs with a feline endogenous retrovirus RD-114
BACKGROUND The feline endogenous retrovirus RD114 is contained in the genome of cats. The virus may contaminate live canine vaccines based on cultured feline cells. The in vivo infectivity, acute and subacute pathogenicity, and viral proliferation of the RD114 virus were evaluated by experimental infection of dogs. METHODS Nine specific pathogen free dogs were divided into three groups, with ...
متن کاملEndogenous feline retrovirus RD-114 does not elicit neutralizing antibodies in dogs.
At least two retroviruses, endogenous feline leukemia virus (FeLV) and RD-114 virus, are assumed to be integrated in the genome of domestic cats (1). Several feline cell lines have been shown to produce RD-114-related viruses (2). In Japan, feline cell lines are widely used to produce vaccines for dogs, particularly against canine parvovirus. The level of infectious RD-114 virus found in canine...
متن کاملStructure, subunit composition, and molecular weight of RD-114 RNA.
The properties and subunit composition of the RNA extracted from RD-114 virions have been studied. The RNA extracted from the virion has a sedimentation coefficient of 52S in a nondenaturing aqueous electrolyte. The estimated molecular weight by sedimentation in nondenaturing and weakly denaturing media is in the range 5.7 X 10(6) to 7.0 X 10(6). By electron microscopy, under moderately denatur...
متن کاملGenetic Diversity and Molecular Phylogeny of Iranian Sheep Based on Cytochrome b Gene Sequences
Phylogenetic relationships and genetic variation between two Iranian sheep breeds were analyzed using cytochrome b (cyt-b) gene sequences. The genomic DNA was isolated by salting out method and amplified cytochrome b gene using polymerase chain reaction restriction (PCR) method with a pair of primer. A partial sequence of cyt-b gene of Iranian sheep is 780 bp and contained 13 variable sites and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virology
سال: 1984
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.52.1.164-171.1984